Journal: Inflammation
Article Title: Piperlongumin Improves Survival in the Mouse Model of Sepsis: Effect on Coagulation Factors and Lung Inflammation
doi: 10.1007/s10753-022-01709-x
Figure Lengend Snippet: PL alleviates inflammation and fibrin deposition in the lungs of CLP mice. A HE-stained images of lungs in each group were observed under an optical microscope (scale bar: 10 µm). B Lung injury score was evaluated according to the degree and scope of lung injury, including the degree of interstitial edema, septal wall thickness, and inflammatory cell infiltration. C Representative images of immunohistochemical staining of fibrin were obtained in the lungs from three groups of mice (400 ×). D The western blot results showed IL-1β, TNF-α, and IL-6 protein levels in lung tissues, and the relative expression levels of IL-1β, TNF-α, and IL-6 normalized to tubulin. Values are shown as mean ± SD. ** P < 0.01 versus sham group; ## P < 0.01 versus CLP group.
Article Snippet: The reagent kits are as follows: IL-6 (70-EK206, Multi Science, China), IL-1β (70-EK201B, Multi Science, China), TNF-α (70-EK282, Multi Science, China), TF (DY3178-05, R&D systems, USA), PAI-1 (DY3828-05 R&D systems, USA), D-dimer (CSB-E13584m, Cusabio, China), fibrinogen (CSB-E08202m, Cusabio, China), and TAT (CSB-E08433m, Cusabio, China).
Techniques: Staining, Microscopy, Immunohistochemical staining, Western Blot, Expressing